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利用功能基因组筛选识别在肿瘤细胞球体培养潜在的新药物靶标
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Utilizing Functional Genomics Screening to Identify Potentially Novel Drug Targets in Cancer Cell Spheroid Cultures
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利用功能基因组筛选识别在肿瘤细胞球体培养潜在的新药物靶标

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07:48 min

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December 26, 2016

DOI:

10.3791/54738-v

DOI:
10.3791/54738-v

•

07:48 min
December 26, 2016

•

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1The Breast Cancer Now Toby Robins Research Centre, Division of Breast Cancer,The Institute of Cancer Research, 2Division of Molecular Pathology,The Institute of Cancer Research, 3Institute of Cancer Sciences,University of Manchester

Tags

Functional GenomicsSiRNA ScreenCancer Cell Spheroid3D CultureHypoxiaTargeted ScreenOncogenesTumor SuppressorsCell ViabilityApoptosisTransfectionCell CultureCell CountingHigh-throughput Screening

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Morrison, E., Wai, P., Leonidou, A., Bland, P., Khalique, S., Farnie, G., Daley, F., Peck, B., Natrajan, R. Utilizing Functional Genomics Screening to Identify Potentially Novel Drug Targets in Cancer Cell Spheroid Cultures. J. Vis. Exp. (118), e54738, doi:10.3791/54738 (2016).

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