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مقايسة على أساس CFSE لدراسة الهجرة من الجلد الفئران الخلايا الجذعية في تصريف الغدد الليمفاوية خلال العدوى مع<em> المتفطرة البقرية</em> عصيات كالميت غيران
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
A CFSE-based Assay to Study the Migration of Murine Skin Dendritic Cells into Draining Lymph Nodes During Infection with Mycobacterium bovis Bacille Calmette-Guérin
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مقايسة على أساس CFSE لدراسة الهجرة من الجلد الفئران الخلايا الجذعية في تصريف الغدد الليمفاوية خلال العدوى مع<em> المتفطرة البقرية</em> عصيات كالميت غيران

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11,784 Views

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07:14 min

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October 09, 2016

DOI:

10.3791/54620-v

DOI:
10.3791/54620-v

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07:14 min
October 09, 2016

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5 Views
, ,
1Department of Microbiology, Tumor and Cell Biology (MTC),Karolinska Institutet

Summary

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An assay in mice to track cell migration from skin to draining lymph node is described which enables the characterization of skin Dendritic cells mobilized to the lymph node after footpad infection with Bacille Calmette-Guérin.

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CFSEDendritic CellsMigrationDraining Lymph NodesMycobacterium Bovis BCGFoot Pad InjectionCell TrackingFlow Cytometry

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Cite this Article

Bollampalli, V. P., Nylén, S., Rothfuchs, A. G. A CFSE-based Assay to Study the Migration of Murine Skin Dendritic Cells into Draining Lymph Nodes During Infection with Mycobacterium bovis Bacille Calmette-Guérin. J. Vis. Exp. (116), e54620, doi:10.3791/54620 (2016).

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