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편광 TIRF 현미경에 의해 감시 미세 유동 흐름 전지에 닿는 지원 이중층 단일 테오의 SNARE 매개 퓨전
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Neuroscience
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JoVE Journal Neuroscience
SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy
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편광 TIRF 현미경에 의해 감시 미세 유동 흐름 전지에 닿는 지원 이중층 단일 테오의 SNARE 매개 퓨전

10,723 Views

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10:58 min

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August 24, 2016

DOI:

10.3791/54349-v

DOI:
10.3791/54349-v

•

10:58 min
August 24, 2016

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10 Views
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1Department of Cellular and Molecular Physiology,Yale University School of Medicine, 2Nanobiology Institute,Yale University, 3Department of Molecular Biophysics and Biochemistry,Yale University, 4Laboratoire de Neurophotonique, Université Paris Descartes, Faculté des Sciences Fondamentales et Biomédicales,Centre National de la Recherche Scientifique (CNRS)

Tags

SNARE-mediatedProteoliposomesSupported BilayersMicrofluidic Flow CellPolarized TIRF MicroscopyMembrane FusionNeurotransmitter ReleaseHormone ReleaseVesicle DockingFusion PoreExocytosisEnvelope VirusesPDMSPhotolithographyMicrofabricationProtein PurificationImaging

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Cite this Article

Nikolaus, J., Karatekin, E. SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy. J. Vis. Exp. (114), e54349, doi:10.3791/54349 (2016).

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