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偏光TIRF顕微鏡によって監視マイクロ流体フローセル内のテザー支持された二分子層​​を持つシングルプロテオリポソー​​ムのSNARE媒介性の融合
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Neuroscience
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JoVE Journal Neuroscience
SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy
Please note that all translations are automatically generated. Click here for the English version.

偏光TIRF顕微鏡によって監視マイクロ流体フローセル内のテザー支持された二分子層​​を持つシングルプロテオリポソー​​ムのSNARE媒介性の融合

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10:58 min

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August 24, 2016

DOI:

10.3791/54349-v

DOI:
10.3791/54349-v

•

10:58 min
August 24, 2016

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10 Views
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1Department of Cellular and Molecular Physiology,Yale University School of Medicine, 2Nanobiology Institute,Yale University, 3Department of Molecular Biophysics and Biochemistry,Yale University, 4Laboratoire de Neurophotonique, Université Paris Descartes, Faculté des Sciences Fondamentales et Biomédicales,Centre National de la Recherche Scientifique (CNRS)

Tags

SNARE-mediatedProteoliposomesSupported BilayersMicrofluidic Flow CellPolarized TIRF MicroscopyMembrane FusionNeurotransmitter ReleaseHormone ReleaseVesicle DockingFusion PoreExocytosisEnvelope VirusesPDMSPhotolithographyMicrofabricationProtein PurificationImaging

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Nikolaus, J., Karatekin, E. SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy. J. Vis. Exp. (114), e54349, doi:10.3791/54349 (2016).

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