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En enkel Flödescytometriska metod för att mäta glukosupptag och glukostransportör uttryck för monocyt Subpopulationer i helblod
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1Centre for Biomedical Research,Macfarlane Burnet Institute for Medical Research and Public Health, 2Department of Infectious Diseases,Monash University, 3Department of Microbiology and Immunology,University of Melbourne, 4Department of Microbiology,The University of the West Indies, 5Division of Experimental Medicine, Department of Medicine,University of California, San Francisco, 6Department of Medicine,Monash University
Chapters
- 00:05Title
- 00:43Monocyte and Monocyte Subpopulation Glucose Transporter 1 (Glut1) Cell Surface Detection
- 02:07Monocyte Glucose Uptake Analysis
- 03:11Data Acquisition and Analysis
- 04:16Results: Representative Monocyte Subpopulation Glucose Uptake Analysis
- 05:22Conclusion
Monocytes are integral components of the human innate immune system that rely on glycolytic metabolism when activated. We describe a flow cytometry protocol to measure glucose transporter expression and glucose uptake by total monocytes and monocyte subpopulations in fresh whole blood.
