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デュアル赤外線蛍光スキャンを用いてラットオリゴデンドロサイト前駆細胞培養およびOligodendrogenesisの定量化の準備
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Developmental Biology
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JoVE Journal Developmental Biology
Preparation of Rat Oligodendrocyte Progenitor Cultures and Quantification of Oligodendrogenesis Using Dual-infrared Fluorescence Scanning
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デュアル赤外線蛍光スキャンを用いてラットオリゴデンドロサイト前駆細胞培養およびOligodendrogenesisの定量化の準備

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11,554 Views

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11:11 min

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February 17, 2016

DOI:

10.3791/53764-v

DOI:
10.3791/53764-v

•

11:11 min
February 17, 2016

•

8 Views
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1Neurology,Johns Hopkins University, School of Medicine

Summary

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Oligodendrocytes are the myelinating cells of the central nervous system. This protocol describes a method for the isolation and culture of their precursors, oligodendrocyte progenitor cells, from rat cortices, as well as a fast and reliable quantitative method to evaluate oligodendrogenesis in vitro in response to experimental factors.

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Cite this Article

Schott, J. T., Kirby, L. A., Calabresi, P. A., Baxi, E. G. Preparation of Rat Oligodendrocyte Progenitor Cultures and Quantification of Oligodendrogenesis Using Dual-infrared Fluorescence Scanning. J. Vis. Exp. (108), e53764, doi:10.3791/53764 (2016).

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