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In Vivo Functional Brain Imaging Approach Based on Bioluminescent Calcium Indicator GFP-aequorin
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1Equipe: Imagerie Cérébrale Fonctionnelle et Comportements (ICFC), Institut des Neurosciences Paris-Saclay (Nero-PSI),UMR-9197, CNRS/Université Paris Sud, 2Interdisciplinary Program in Neuroscience, Graduate College,University of Iowa, 3Department of Anesthesia, Carver College of Medicine,University of Iowa
Chapters
- 00:05Title
- 00:57Positioning and Gluing of Drosophila
- 02:23Drosophila Dissection
- 03:58Imaging Setup and Sample Preparation
- 05:47Image Recording
- 07:39Extracting Photon Values
- 09:51Results: In Vivo Functional Brain Imaging
- 11:00Conclusion
Here we present a novel Ca2+-imaging approach using a bioluminescent reporter. This approach uses a fused construct GFP-aequorin which binds to Ca2+ and emits light, eliminating the need for light excitation. Significantly this method permits long continuous imaging, access to deep brain structures and high temporal resolution.
