Journal
/
Developmental Biology
/
可溶性タバコの煙抽出物によって損傷した後のマウス間葉系幹細胞の移動の変化を検出するために創傷スクラッチアッセイの最適化
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Optimization of the Wound Scratch Assay to Detect Changes in Murine Mesenchymal Stromal Cell Migration After Damage by Soluble Cigarette Smoke Extract
Please note that all translations are automatically generated. Click here for the English version.

可溶性タバコの煙抽出物によって損傷した後のマウス間葉系幹細胞の移動の変化を検出するために創傷スクラッチアッセイの最適化

日本語

Automatically Generated

16,754 Views

•

08:40 min

•

December 03, 2015

DOI:

10.3791/53414-v

DOI:
10.3791/53414-v

•

08:40 min
December 03, 2015

•

2 Views
, , ,
1Department of Biology,College of the Holy Cross

Summary

Automatically generated

The capacity to migrate is a key function of many different cell types, including mesenchymal stromal cells (MSCs). However, quantifying alterations in migratory capacity after damage is challenging. This protocol describes an easily adaptable migration assay that uses rigorous statistics to quantify changes in MSC migratory capacity after damage.

Explore More Videos

Optimized Wound Scratch AssayMesenchymal Stromal Cell MigrationQuantitative Migration AnalysisSoluble Cigarette Smoke ExtractCell Migration AssayScratch Assay OptimizationBoyden ChamberCell Exclusion Zone AssayCell Migration QuantificationReproducibilityCustomizabilityCost-effectiveness

Read Article

Cite this Article

Cormier, N., Yeo, A., Fiorentino, E., Paxson, J. Optimization of the Wound Scratch Assay to Detect Changes in Murine Mesenchymal Stromal Cell Migration After Damage by Soluble Cigarette Smoke Extract. J. Vis. Exp. (106), e53414, doi:10.3791/53414 (2015).

Download .ris file

Copy

Share Video

.

Copy