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Optimierung der Wund Scratch Assay auf Änderungen in Murine mesenchymale Stromazellen Zellmigration Detect nach der Beschädigung durch lösliche Cigarette Smoke Extract
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Developmental Biology
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JoVE Journal Developmental Biology
Optimization of the Wound Scratch Assay to Detect Changes in Murine Mesenchymal Stromal Cell Migration After Damage by Soluble Cigarette Smoke Extract
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Optimierung der Wund Scratch Assay auf Änderungen in Murine mesenchymale Stromazellen Zellmigration Detect nach der Beschädigung durch lösliche Cigarette Smoke Extract

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16,754 Views

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08:40 min

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December 03, 2015

DOI:

10.3791/53414-v

DOI:
10.3791/53414-v

•

08:40 min
December 03, 2015

•

2 Views
, , ,
1Department of Biology,College of the Holy Cross

Summary

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The capacity to migrate is a key function of many different cell types, including mesenchymal stromal cells (MSCs). However, quantifying alterations in migratory capacity after damage is challenging. This protocol describes an easily adaptable migration assay that uses rigorous statistics to quantify changes in MSC migratory capacity after damage.

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Optimized Wound Scratch AssayMesenchymal Stromal Cell MigrationQuantitative Migration AnalysisSoluble Cigarette Smoke ExtractCell Migration AssayScratch Assay OptimizationBoyden ChamberCell Exclusion Zone AssayCell Migration QuantificationReproducibilityCustomizabilityCost-effectiveness

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Cite this Article

Cormier, N., Yeo, A., Fiorentino, E., Paxson, J. Optimization of the Wound Scratch Assay to Detect Changes in Murine Mesenchymal Stromal Cell Migration After Damage by Soluble Cigarette Smoke Extract. J. Vis. Exp. (106), e53414, doi:10.3791/53414 (2015).

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