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גאומגנטית שדה (GMF) וצמח אבולוציה: לחקור את ההשפעות של ביטול GMF ב<em> Thaliana ארבידופסיס</em> ביטוי פיתוח וג'ין
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Geomagnetic Field (Gmf) and Plant Evolution: Investigating the Effects of Gmf Reversal on Arabidopsis thaliana Development and Gene Expression
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גאומגנטית שדה (GMF) וצמח אבולוציה: לחקור את ההשפעות של ביטול GMF ב<em> Thaliana ארבידופסיס</em> ביטוי פיתוח וג'ין

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12,648 Views

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11:04 min

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November 30, 2015

DOI:

10.3791/53286-v

DOI:
10.3791/53286-v

•

11:04 min
November 30, 2015

•

87 Views
, , , ,
1Department of Life Sciences and Systems Biology,University of Turin, 2Radcliffe Department of Medicine,University of Oxford, John Radcliffe Hospital

Summary

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This protocol describes a method to test the hypothesis that the reversal of the effective geomagnetic field (GMF) induces differential gene expression and alters the morphology of Arabidopsis thaliana. A triaxial octagonal system of Helmholtz coil-pairs was used to artificially generate reversed GMF conditions in the plant growth chamber.

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Geomagnetic Field (GMF)GMF ReversalArabidopsis ThalianaPlant DevelopmentGene ExpressionHelmholtz CoilsQPCRCRU3COTP1RRTF1FSD1CAT3TAPXAPX1RbohD

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Cite this Article

Bertea, C. M., Narayana, R., Agliassa, C., Rodgers, C. T., Maffei, M. E. Geomagnetic Field (Gmf) and Plant Evolution: Investigating the Effects of Gmf Reversal on Arabidopsis thaliana Development and Gene Expression. J. Vis. Exp. (105), e53286, doi:10.3791/53286 (2015).

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