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Generación de células madre pluripotentes inducidas a partir de Frozen Buffy Coats utilizando para no integrar episomal plásmidos
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Developmental Biology
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JoVE Journal Developmental Biology
Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids
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Generación de células madre pluripotentes inducidas a partir de Frozen Buffy Coats utilizando para no integrar episomal plásmidos

español

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12,703 Views

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10:52 min

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June 05, 2015

DOI:

10.3791/52885-v

DOI:
10.3791/52885-v

•

10:52 min
June 05, 2015

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5 Views
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1Center for Biomedicine,European Academy Bozen/Bolzano (EURAC), 2Laboratory of Medical Genetics,Fondazione IRCCS Ca´ Granda, Ospedale Maggiore Policlinico, 3Del E. Webb Center for Neuroscience, Aging & Stem Cell Research,Sanford-Burnham Medical Research Institute

Summary

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Las células madre pluripotentes inducidas (iPSCs) representan una fuente de tejidos específicos del paciente para aplicaciones clínicas y de investigación básica. A continuación, presentamos un protocolo detallado para reprogramar células humanas mononucleares de sangre periférica (PBMNCs) obtenidos a partir de buffy abrigos congelados en CMPI-virales gratis utilizando no integración de plásmidos episomales.

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Induced Pluripotent Stem CellsIPSCsNon-integrating Episomal PlasmidsReprogrammingPeripheral Blood Mononuclear CellsPBMNCsFrozen Buffy CoatsRegenerative MedicinePatient-specificTransgene-free

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Meraviglia, V., Zanon, A., Lavdas, A. A., Schwienbacher, C., Silipigni, R., Di Segni, M., Chen, H. V., Pramstaller, P. P., Hicks, A. A., Rossini, A. Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids. J. Vis. Exp. (100), e52885, doi:10.3791/52885 (2015).

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