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Immunology and Infection

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生成和多表型高内涵筛选<em>贝氏柯克斯体</em>转座子突变体

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Generation and Multi-phenotypic High-content Screening of Coxiella burnetii Transposon Mutants

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生成和多表型高内涵筛选<em>贝氏柯克斯体</em>转座子突变体

9,489 Views

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11:44 min

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May 13, 2015

DOI:

10.3791/52851-v

DOI:

10.3791/52851-v

•

11:44 min

May 13, 2015

•

1 Views

Eric Martinez¹, Franck Cantet¹, Matteo Bonazzi¹

¹Cell Biology of Bacterial Infections, CNRS, FRE3689, CPBS,Université Montpellier

Summary

贝氏柯克斯体是专性细胞内革兰阴性细菌负责对人畜共患病Q热。在这里，我们描述了用于Coxiella荧光转座子突变体的产生，以及自动识别和所得内化，复制和细胞毒性表型分析的方法。

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