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November 10, 2015
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1Department of Organismic and Evolutionary Biology,Harvard University, 2Department of Immunology and Infectious Diseases,Harvard T.H. Chan School of Public Health, 3Faculty of Medicine and Pharmacy,Cheikh Anta Diop University, 4School of Nursing and Health Sciences,Simmons College, 5Institute of Infectious Diseases,Broad Institute
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虽然高分辨率熔解分析提供了一个异质群体的单核苷酸多态性区分的能力，突变等位基因扩增偏置可提高其检测存在于样品中相对较低的百分比等位基因的能力。本协议描述的改进，提高了高分辨率熔解分析的灵敏度。
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Daniels, R., Hamilton, E. J., Durfee, K., Ndiaye, D., Wirth, D. F., Hartl, D. L., Volkman, S. K. Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria. J. Vis. Exp. (105), e52839, doi:10.3791/52839 (2015).
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