/
/
Un microbienne aquatique métaprotéomique Workflow: à partir de cellules à tryptique peptides appropriés pour l'analyse comparative-spectrométrie de masse en tandem
A subscription to JoVE is required to view this content. Sign in or start your free trial.
8,639 Views
•
08:09 min
•
September 15, 2015
DOI:
Summary
Automatically generated
This protocol is for the extraction and concentration of protein and DNA from microbial biomass collected from seawater, followed by the generation of tryptic peptides suitable for tandem mass spectrometry-based proteomic analysis.
Read Article
Cite this Article
Colatriano, D., Walsh, D. A. An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis. J. Vis. Exp. (103), e52827, doi:10.3791/52827 (2015).
Copy