Journal
/
Environment
/
Een Aquatic Microbial MetaProteomics Workflow: Van cellen tot Tryptic Peptiden geschikt voor Tandem massaspectrometrie-gebaseerde Analyse
JoVE Journal
Environment
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Environment
An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis
Please note that all translations are automatically generated. Click here for the English version.

Een Aquatic Microbial MetaProteomics Workflow: Van cellen tot Tryptic Peptiden geschikt voor Tandem massaspectrometrie-gebaseerde Analyse

Nederlands

Automatically Generated

8,640 Views

•

08:09 min

•

September 15, 2015

DOI:

10.3791/52827-v

DOI:
10.3791/52827-v

•

08:09 min
September 15, 2015

•

3 Views
,
1Department of Biology,Concordia University

Summary

Automatically generated

This protocol is for the extraction and concentration of protein and DNA from microbial biomass collected from seawater, followed by the generation of tryptic peptides suitable for tandem mass spectrometry-based proteomic analysis.

Explore More Videos

Aquatic Microbial MetaproteomicsMetaproteomicsMetagenomicsMetatranscriptomicsMarine Microbial CellsSterivex FilterRNA StabilizationPeptide DigestionTandem Mass SpectrometryDatabase Search

Read Article

Cite this Article

Colatriano, D., Walsh, D. A. An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis. J. Vis. Exp. (103), e52827, doi:10.3791/52827 (2015).

Download .ris file

Copy

Share Video

.

Copy