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والمائية الميكروبية Metaproteomics سير العمل: من الخلايا إلى زيتية الببتيدات مناسبة لتحليل يستند الطيف، جنبا إلى جنب الشامل
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An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis
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والمائية الميكروبية Metaproteomics سير العمل: من الخلايا إلى زيتية الببتيدات مناسبة لتحليل يستند الطيف، جنبا إلى جنب الشامل

العربية

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8,640 Views

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08:09 min

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September 15, 2015

DOI:

10.3791/52827-v

DOI:
10.3791/52827-v

•

08:09 min
September 15, 2015

•

3 Views
,
1Department of Biology,Concordia University

Summary

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This protocol is for the extraction and concentration of protein and DNA from microbial biomass collected from seawater, followed by the generation of tryptic peptides suitable for tandem mass spectrometry-based proteomic analysis.

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Colatriano, D., Walsh, D. A. An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis. J. Vis. Exp. (103), e52827, doi:10.3791/52827 (2015).

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