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Isolering og karakterisering af satellit-celler fra rotte Hoved Branchiomeric Muskler
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1Department of Orthodontics and Craniofacial Biology, Radboud Institute for Molecular Life Sciences,Radboud University Medical Center, 2Department of Biological Structure,University of Washington School of Medicine, 3Department of Biochemistry, Radboud Institute for Molecular Life Sciences,Radboud University Medical Center
Chapters
- 00:05Title
- 01:33Head Muscle (Masseter, Digastric, and Levator Veli Palatini) Dissection
- 04:01Satellite Cell Isolation and Culture
- 06:18Results: Representative Myotube Generation from Rat Head Branchiometric Muscle Tissue
- 07:09Conclusion
Denne protokol beskriver isoleringen af satellit celler fra branchiomeric hoved muskler en 9 uger gammel rotte. Musklerne stammer fra forskellige Branchialfodder buer. Efterfølgende satellit-celler dyrkes på en plet belægning af millimeter størrelse at studere deres differentiering. Denne fremgangsmåde undgår udvidelse og passage af satellit celler.
