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Automated Gel seleção de tamanho para melhorar a qualidade das bibliotecas de sequenciação de próxima geração Preparado a partir de amostras de água do ambiente
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JoVE Journal Environment
Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

Automated Gel seleção de tamanho para melhorar a qualidade das bibliotecas de sequenciação de próxima geração Preparado a partir de amostras de água do ambiente

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10,403 Views

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13:26 min

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April 17, 2015

DOI:

10.3791/52685-v

DOI:
10.3791/52685-v

•

13:26 min
April 17, 2015

•

3 Views
, , , , , ,
1Department of Pathology and Laboratory Medicine, Faculty of Medicine,The University of British Columbia, 2Coastal Genomics, 3British Columbia Public Health Microbiology and Reference Laboratory

Summary

Automatically generated

This manuscript describes an automated gel size selection approach for purifying DNA fragments for next-generation sequencing. The Ranger Technology provides complete automation of the entire process of agarose gel loading, electrophoretic analysis, and recovery of targeted DNA fragments allowing for high-throughput and high quality next-generation sequencing libraries.

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Keyword Extraction:Next-generation SequencingEnvironmental Water SamplesDNA Library PreparationGel Size SelectionAutomated ElectrophoresisRanger TechnologyRead LengthRead Quality

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Uyaguari-Diaz, M. I., Slobodan, J. R., Nesbitt, M. J., Croxen, M. A., Isaac-Renton, J., Prystajecky, N. A., Tang, P. Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples. J. Vis. Exp. (98), e52685, doi:10.3791/52685 (2015).

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