Journal
/
Environment
/
Sélection de la taille Gel automatisé pour améliorer la qualité des bibliothèques de séquençage de prochaine génération Préparé à partir d'échantillons d'eau de l'environnement
JoVE Journal
Environment
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Environment
Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples
Please note that all translations are automatically generated. Click here for the English version.

Sélection de la taille Gel automatisé pour améliorer la qualité des bibliothèques de séquençage de prochaine génération Préparé à partir d'échantillons d'eau de l'environnement

français

Automatically Generated

10,403 Views

•

13:26 min

•

April 17, 2015

DOI:

10.3791/52685-v

DOI:
10.3791/52685-v

•

13:26 min
April 17, 2015

•

3 Views
, , , , , ,
1Department of Pathology and Laboratory Medicine, Faculty of Medicine,The University of British Columbia, 2Coastal Genomics, 3British Columbia Public Health Microbiology and Reference Laboratory

Summary

Automatically generated

This manuscript describes an automated gel size selection approach for purifying DNA fragments for next-generation sequencing. The Ranger Technology provides complete automation of the entire process of agarose gel loading, electrophoretic analysis, and recovery of targeted DNA fragments allowing for high-throughput and high quality next-generation sequencing libraries.

Explore More Videos

Keyword Extraction:Next-generation SequencingEnvironmental Water SamplesDNA Library PreparationGel Size SelectionAutomated ElectrophoresisRanger TechnologyRead LengthRead Quality

Read Article

Cite this Article

Uyaguari-Diaz, M. I., Slobodan, J. R., Nesbitt, M. J., Croxen, M. A., Isaac-Renton, J., Prystajecky, N. A., Tang, P. Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples. J. Vis. Exp. (98), e52685, doi:10.3791/52685 (2015).

Download .ris file

Copy

Share Video

.

Copy