3D organotypiques Co-culture Modèle Soutenir médullaire thymique des cellules épithéliales prolifération, la différenciation et Promiscuous Gene Expression
3D organotypiques Co-culture Modèle Soutenir médullaire thymique des cellules épithéliales prolifération, la différenciation et Promiscuous Gene Expression
Studying medullary thymic epithelial cells in vitro has been largely unsuccessful, as current 2D culture systems do not mimic the in vivo scenario. The 3D culture system described herein - a modified skin organotypic culture model - has proven superior in recapitulating mTEC proliferation, differentiation and maintenance of promiscuous gene expression.