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Functional Evaluation of Biological Neurotoxins in Networked Cultures of Stem Cell-derived Central Nervous System Neurons
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Functional Evaluation of Biological Neurotoxins in Networked Cultures of Stem Cell-derived Central Nervous System Neurons

Functional Evaluation of Biological Neurotoxins in Networked Cultures of Stem Cell-derived Central Nervous System Neurons

English

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9,066 Views

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15:05 min

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February 05, 2015

DOI:

10.3791/52361-v

DOI:
10.3791/52361-v

•

15:05 min
February 05, 2015

•

2 Views
, , ,
1Research Division, Cellular Molecular Biology Branch,United States Army Medical Research Institute of Chemical Defense

Summary

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A custom protocol is described to differentiate mouse ES cells into defined populations of highly pure neurons exhibiting functioning synapses and emergent network behavior. Electrophysiological analysis demonstrates the loss of synaptic transmission following exposure to botulinum neurotoxin serotypes /A-/G and tetanus neurotoxin.

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Stem Cell-derived NeuronsCentral Nervous SystemNeurotoxinsClostridial NeurotoxinsBotulinum NeurotoxinTetanus NeurotoxinSynaptic ActivityElectrophysiologyMiniature Excitatory Post-synaptic CurrentsDose-dependent EffectsIn Vitro Model

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Cite this Article

Hubbard, K., Beske, P., Lyman, M., McNutt, P. Functional Evaluation of Biological Neurotoxins in Networked Cultures of Stem Cell-derived Central Nervous System Neurons. J. Vis. Exp. (96), e52361, doi:10.3791/52361 (2015).

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