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生物神经毒素在茎的网络文化功能评估细胞衍生的中枢神经系统神经元
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Functional Evaluation of Biological Neurotoxins in Networked Cultures of Stem Cell-derived Central Nervous System Neurons
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生物神经毒素在茎的网络文化功能评估细胞衍生的中枢神经系统神经元

English

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9,055 Views

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15:05 min

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February 05, 2015

DOI:

10.3791/52361-v

DOI:
10.3791/52361-v

•

15:05 min
February 05, 2015

•

2 Views
, , ,
1Research Division, Cellular Molecular Biology Branch,United States Army Medical Research Institute of Chemical Defense

Summary

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A custom protocol is described to differentiate mouse ES cells into defined populations of highly pure neurons exhibiting functioning synapses and emergent network behavior. Electrophysiological analysis demonstrates the loss of synaptic transmission following exposure to botulinum neurotoxin serotypes /A-/G and tetanus neurotoxin.

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Stem Cell-derived NeuronsCentral Nervous SystemNeurotoxinsClostridial NeurotoxinsBotulinum NeurotoxinTetanus NeurotoxinSynaptic ActivityElectrophysiologyMiniature Excitatory Post-synaptic CurrentsDose-dependent EffectsIn Vitro Model

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Cite this Article

Hubbard, K., Beske, P., Lyman, M., McNutt, P. Functional Evaluation of Biological Neurotoxins in Networked Cultures of Stem Cell-derived Central Nervous System Neurons. J. Vis. Exp. (96), e52361, doi:10.3791/52361 (2015).

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