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Måling Protein Stabilitet på Living Sebrafisk embryo med fluoresens Decay Etter Photoconversion (FDAP)
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Developmental Biology
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JoVE Journal Developmental Biology
Measuring Protein Stability in Living Zebrafish Embryos Using Fluorescence Decay After Photoconversion (FDAP)

Måling Protein Stabilitet på Living Sebrafisk embryo med fluoresens Decay Etter Photoconversion (FDAP)

11,066 Views

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09:45 min

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January 28, 2015

DOI:

10.3791/52266-v

DOI:
10.3791/52266-v

•

09:45 min
January 28, 2015

•

5 Views
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1Department of Molecular and Cellular Biology,Harvard University, 2Systems Biology of Development Group,Friedrich Miescher Laboratory of the Max Planck Society

Tags

Protein StabilityFluorescence Decay After PhotoconversionFDAPProtein Clearance KineticsPhotoconvertible Fluorescent ProteinZebrafish EmbryosCompartmental AnalysisIntra- And Extracellular Half-livesSecreted Signaling ProteinsIn Vivo Protein Dynamics

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Rogers, K. W., Bläßle, A., Schier, A. F., Müller, P. Measuring Protein Stability in Living Zebrafish Embryos Using Fluorescence Decay After Photoconversion (FDAP). J. Vis. Exp. (95), e52266, doi:10.3791/52266 (2015).

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