Journal
/
Developmental Biology
/
Photoconversion के बाद प्रतिदीप्ति क्षय का प्रयोग लिविंग zebrafish भ्रूण में प्रोटीन स्थिरता मापने (FDAP)
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Measuring Protein Stability in Living Zebrafish Embryos Using Fluorescence Decay After Photoconversion (FDAP)
Please note that all translations are automatically generated. Click here for the English version.

Photoconversion के बाद प्रतिदीप्ति क्षय का प्रयोग लिविंग zebrafish भ्रूण में प्रोटीन स्थिरता मापने (FDAP)

11,066 Views

•

09:45 min

•

January 28, 2015

DOI:

10.3791/52266-v

DOI:
10.3791/52266-v

•

09:45 min
January 28, 2015

•

5 Views
, , ,
1Department of Molecular and Cellular Biology,Harvard University, 2Systems Biology of Development Group,Friedrich Miescher Laboratory of the Max Planck Society

Tags

Protein StabilityFluorescence Decay After PhotoconversionFDAPProtein Clearance KineticsPhotoconvertible Fluorescent ProteinZebrafish EmbryosCompartmental AnalysisIntra- And Extracellular Half-livesSecreted Signaling ProteinsIn Vivo Protein Dynamics

Read Article

Cite this Article

Rogers, K. W., Bläßle, A., Schier, A. F., Müller, P. Measuring Protein Stability in Living Zebrafish Embryos Using Fluorescence Decay After Photoconversion (FDAP). J. Vis. Exp. (95), e52266, doi:10.3791/52266 (2015).

Download .ris file

Copy

Share Video

.

Copy