Journal
/
Chemistry
/
Fluoreszenz-basierte Überwachung von PAD4 Aktivität über eine Pro-Fluoreszenz Substratanalogon
JoVE Journal
Chemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Chemistry
Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog
Please note that all translations are automatically generated. Click here for the English version.

Fluoreszenz-basierte Überwachung von PAD4 Aktivität über eine Pro-Fluoreszenz Substratanalogon

Deutsch

Automatically Generated

9,718 Views

•

08:37 min

•

November 05, 2014

DOI:

10.3791/52114-v

DOI:
10.3791/52114-v

•

08:37 min
November 05, 2014

•

10 Views
, ,
1Department of Chemistry,Lehigh University

Summary

Automatically generated

PAD4 is an enzyme responsible for the conversion of peptidyl-arginine to peptidyl-citrulline. Dysregulation of PAD4 has been implicated in a number of human diseases. A facile and high-throughput compatible fluorescence based PAD4 assay is described.

Explore More Videos

Fluorescence-based AssayPAD4 ActivityPro-fluorescence Substrate AnalogPost-translational ModificationsHistone TailsArginine To Citrulline ConversionHigh-throughput ScreeningCell LysatesPAD4 Over-expression

Read Article

Cite this Article

Sabulski, M. J., Fura, J. M., Pires, M. M. Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog. J. Vis. Exp. (93), e52114, doi:10.3791/52114 (2014).

Download .ris file

Copy

Share Video

.

Copy