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애벌레 Zebrafish의 피부에 유도 GAL4 / UAS 식 시스템을 사용하여 선천성 면역과 종양 전 세포 상호 작용의 라이브 영상
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Live Imaging of Innate Immune and Preneoplastic Cell Interactions Using an Inducible Gal4/UAS Expression System in Larval Zebrafish Skin
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애벌레 Zebrafish의 피부에 유도 GAL4 / UAS 식 시스템을 사용하여 선천성 면역과 종양 전 세포 상호 작용의 라이브 영상

English

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11,664 Views

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08:52 min

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February 03, 2015

DOI:

10.3791/52107-v

DOI:
10.3791/52107-v

•

08:52 min
February 03, 2015

•

2 Views
, , , ,
1MRC Centre for Inflammation Research, Queen’s Medical Research Institute,The University of Edinburgh, 2Department of Cell & Developmental Biology,University College London

Summary

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Studying the earliest events of preneoplastic cell progression and innate immune cell interaction is pivotal to understand and treat cancer. Here we describe a method to conditionally induce epithelial cell transformations and the subsequent live imaging of innate immune cell interaction with HRASG12V expressing skin cells in zebrafish larvae.

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Ramezani, T., Laux, D. W., Bravo, I. R., Tada, M., Feng, Y. Live Imaging of Innate Immune and Preneoplastic Cell Interactions Using an Inducible Gal4/UAS Expression System in Larval Zebrafish Skin. J. Vis. Exp. (96), e52107, doi:10.3791/52107 (2015).

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