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先天免疫和癌前细胞间的相互作用使用可诱导的Gal4 / UAS表达系统在斑马鱼幼体皮肤实时成像
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Live Imaging of Innate Immune and Preneoplastic Cell Interactions Using an Inducible Gal4/UAS Expression System in Larval Zebrafish Skin
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先天免疫和癌前细胞间的相互作用使用可诱导的Gal4 / UAS表达系统在斑马鱼幼体皮肤实时成像

English

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11,664 Views

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08:52 min

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February 03, 2015

DOI:

10.3791/52107-v

DOI:
10.3791/52107-v

•

08:52 min
February 03, 2015

•

2 Views
, , , ,
1MRC Centre for Inflammation Research, Queen’s Medical Research Institute,The University of Edinburgh, 2Department of Cell & Developmental Biology,University College London

Summary

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研究癌前细胞发展和先天免疫细胞相互作用的最早事件是举足轻重的理解和治疗癌症。在这里，我们描述了一个方法，有条件地诱导上皮细胞转化和先天免疫细胞相互作用与HRAS G12V表达的皮肤细胞在斑马鱼幼虫随后的实时成像。

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Cite this Article

Ramezani, T., Laux, D. W., Bravo, I. R., Tada, M., Feng, Y. Live Imaging of Innate Immune and Preneoplastic Cell Interactions Using an Inducible Gal4/UAS Expression System in Larval Zebrafish Skin. J. Vis. Exp. (96), e52107, doi:10.3791/52107 (2015).

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