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Isolering og kvantitativ Immuncytokemisk karakterisering af primære myogene celler og fibroblaster fra human skeletmuskel
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Developmental Biology
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JoVE Journal Developmental Biology
Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle

Isolering og kvantitativ Immuncytokemisk karakterisering af primære myogene celler og fibroblaster fra human skeletmuskel

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11:22 min

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January 12, 2015

DOI:

10.3791/52049-v

DOI:
10.3791/52049-v

•

11:22 min
January 12, 2015

•

4 Views
, , , ,
1Centre of Human and Aerospace Physiological Sciences,King’s College London, 2Wellcome Trust-Medical Research Council,Cambridge Stem Cell Institute

Tags

Skeletal MuscleSatellite CellsMyogenic CellsMuscle Precursor CellsFibroblastsCD56DesminTE-7Enzymatic DigestionCollagenaseDispaseMagnetic Activated Cell Sorting (MACS)ImmunocytochemistryCell CultureMuscle Regeneration

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Agley, C. C., Rowlerson, A. M., Velloso, C. P., Lazarus, N. L., Harridge, S. D. R. Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle. J. Vis. Exp. (95), e52049, doi:10.3791/52049 (2015).

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