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October 16, 2014
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The expression of recombinant proteins by mammalian systems is becoming an attractive method for producing protein complexes for structural biology. Here we present a simple yet highly efficient expression system using suspension grown mammalian cells to purify protein complexes for structural studies.
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Portolano, N., Watson, P. J., Fairall, L., Millard, C. J., Milano, C. P., Song, Y., Cowley, S. M., Schwabe, J. W. Recombinant Protein Expression for Structural Biology in HEK 293F Suspension Cells: A Novel and Accessible Approach. J. Vis. Exp. (92), e51897, doi:10.3791/51897 (2014).
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