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Rapid Identifikation af Gram-negative bakterier fra Blood kulturvæsken Brug MALDI-TOF-massespektrometri
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1Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research,Westmead Hospital, 2Centre for Research Excellence in Critical Infection, Westmead Millennium Institute,Westmead Hospital, 3Sydney Emerging Infectious Diseases Institute, University of Sydney,Westmead Hospital
Chapters
- 00:05Title
- 01:20Preparation of Bacterial Inoculate from Blood Culture Broth
- 05:46Preparation of MALDI-TOF Target Plate
- 06:34MALDI-TOF MS Spectra Acquisition
- 08:42Post Analysis-Interpretation of MALDI-TOF MS Scores
- 10:18Representative MALDI-TOF Analysis of Gram Negative Bacteria from Blood Cultures
- 12:35Conclusion
Anvendelsen af matrix-assisteret laser desorption / ionisering tid søgning (MALDI-TOF) massespektrometri (MS) direkte til blod dyrkningssuppe fremskynder identifikation af bakterier. I denne procedure er en hurtig og pålidelig metode til identifikation af Gram-negative bakterier direkte fra blod dyrkningsvæsken.
Tags
Rapid IdentificationGram Negative BacteriaBlood Culture BrothMALDI-TOF Mass SpectrometryClinical MicrobiologyBacterial IdentificationBloodstream InfectionSub-cultureSolid AgarMALDI-TOF MSBlood Culture SpecimensInterfering ResinsProteinsHemoglobinBacterial ConcentrationBacterial PurificationBacterial Extraction
