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行動経験に続いて脳試料からの転写動態の網羅的解析
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Behavior
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JoVE Journal Behavior
Comprehensive Analysis of Transcription Dynamics from Brain Samples Following Behavioral Experience
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行動経験に続いて脳試料からの転写動態の網羅的解析

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11,465 Views

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08:14 min

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August 26, 2014

DOI:

10.3791/51642-v

DOI:
10.3791/51642-v

•

08:14 min
August 26, 2014

•

1 Views
, , , ,
1The Alexander Silberman Institute of Life Sciences & Edmond and Lily Safra Center for Brain Sciences,The Hebrew University of Jerusalem

Summary

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This manuscript describes a protocol that applies comprehensive profiling for analysis of transcriptional programs induced in specific brain nuclei of rodents following behavioral paradigms. Herein, this approach is illustrated in the context of profiling genes induced in the nucleus accumbens (NAc) of mice following acute cocaine exposure, utilizing microfluidic qPCR arrays.

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Transcription DynamicsBrain SamplesBehavioral ExperienceGene ExpressionMolecular NeuroscienceNeuropsychiatric DisordersTranscriptional ProgramsNucleus AccumbensCocaine ExperienceRNA PurificationReverse TranscriptionMicrofluidic ArraysQPCR AnalysisSingle CellsWhole-genome StudiesMicroarraysRNAseq

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Cite this Article

Turm, H., Mukherjee, D., Haritan, D., Tahor, M., Citri, A. Comprehensive Analysis of Transcription Dynamics from Brain Samples Following Behavioral Experience. J. Vis. Exp. (90), e51642, doi:10.3791/51642 (2014).

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