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Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology
JoVE Journal
Biology
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JoVE Journal Biology
Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology

Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology

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11:16 min

July 12, 2014

DOI:

11:16 min
July 12, 2014

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Summary

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HaloTag technology is a multifunctional technology which has shown significant success in isolation of both small and large protein complexes from mammalian cells.  Here we highlight the advantages of this technology compared to existing alternatives and demonstrate its utility to study numerous aspects of protein function inside eukaryotic cells.

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