HaloTag technology is a multifunctional technology which has shown significant success in isolation of both small and large protein complexes from mammalian cells. Here we highlight the advantages of this technology compared to existing alternatives and demonstrate its utility to study numerous aspects of protein function inside eukaryotic cells.
Daniels, D. L., Méndez, J., Benink, H., Niles, A., Murphy, N., Ford, M., Jones, R., Amunugama, R., Allen, D., Urh, M. Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology. J. Vis. Exp. (89), e51553, doi:10.3791/51553 (2014).