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从海马CA1区片核富含分的分离，研究突触核信使蛋白质的活性依赖进口核
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Neuroscience
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JoVE Journal Neuroscience
Isolation of CA1 Nuclear Enriched Fractions from Hippocampal Slices to Study Activity-dependent Nuclear Import of Synapto-nuclear Messenger Proteins
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从海马CA1区片核富含分的分离，研究突触核信使蛋白质的活性依赖进口核

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10:03 min

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August 10, 2014

DOI:

10.3791/51310-v

DOI:
10.3791/51310-v

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10:03 min
August 10, 2014

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1RG Neuroplasticity,Leibniz Institute for Neurobiology, 2Department of Cell Biology,Utrecht University

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Nuclear Enriched FractionHippocampal SlicesSynaptic PlasticityLong-term PotentiationLong-term DepressionProtein TranslocationImmunoblottingActivity-dependentSignaling CascadesCA1 NeuronsERK1/2Jacob

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Yuanxiang, P., Bera, S., Karpova, A., Kreutz, M. R., Mikhaylova, M. Isolation of CA1 Nuclear Enriched Fractions from Hippocampal Slices to Study Activity-dependent Nuclear Import of Synapto-nuclear Messenger Proteins. J. Vis. Exp. (90), e51310, doi:10.3791/51310 (2014).

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