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无细胞的转录和翻译的机械囊泡的封装建设模拟蜂窝
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
The Encapsulation of Cell-free Transcription and Translation Machinery in Vesicles for the Construction of Cellular Mimics
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无细胞的转录和翻译的机械囊泡的封装建设模拟蜂窝

14,695 Views

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10:28 min

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October 21, 2013

DOI:

10.3791/51304-v

DOI:
10.3791/51304-v

•

10:28 min
October 21, 2013

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5 Views
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1Centre for Integrative Biology,University of Trento

Tags

Cell-free TranscriptionCell-free TranslationVesiclesCellular MimicsBottom-upCompartmentalizationWater-in-oil EmulsionsMicrofluidicsPhospholipid VesiclesRecombinant Protein ExpressionGenetic CircuitryMinimum Requirements For Cellular Life

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Spencer, A. C., Torre, P., Mansy, S. S. The Encapsulation of Cell-free Transcription and Translation Machinery in Vesicles for the Construction of Cellular Mimics. J. Vis. Exp. (80), e51304, doi:10.3791/51304 (2013).

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