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In vitro onderzoek naar de impact van immunoregulatoire Pathways op HIV-specifieke CD4 T-cellen Effectorfunctiemanipulatie Evalueer
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Immunology and Infection
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JoVE Journal Immunology and Infection
In Vitro Assay to Evaluate the Impact of Immunoregulatory Pathways on HIV-specific CD4 T Cell Effector Function
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In vitro onderzoek naar de impact van immunoregulatoire Pathways op HIV-specifieke CD4 T-cellen Effectorfunctiemanipulatie Evalueer

13,952 Views

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09:26 min

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October 15, 2013

DOI:

10.3791/50821-v

DOI:
10.3791/50821-v

•

09:26 min
October 15, 2013

•

6 Views
, , , ,
1The Ragon Institute of MGH, MIT and Harvard, 2Centre de Recherche du Centre Hospitalier de l’Université de Montréal (CRCHUM)

Tags

In Vitro AssayHIVCD4 T CellsEffector FunctionImmunoregulatory PathwaysPD-1IL-10T Cell ExhaustionCytokine ProductionPBMCFlow CytometryQRT-PCRGag Peptide Pool

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Porichis, F., Hart, M. G., Zupkosky, J., Barblu, L., Kaufmann, D. E. In Vitro Assay to Evaluate the Impact of Immunoregulatory Pathways on HIV-specific CD4 T Cell Effector Function. J. Vis. Exp. (80), e50821, doi:10.3791/50821 (2013).

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