Afferent sensory neurons signal sensory information from the periphery to the central nervous system. Identifying specific afferent neurons will help in understanding their physiology. We describe a method of retrograde labeling to identify afferent neurons, and study the voltage-gated ion channels in these neurons using patch clamp electrophysiology and immunocytochemistry.
Ramachandra, R., McGrew, S., Elmslie, K. Identification of Specific Sensory Neuron Populations for Study of Expressed Ion Channels. J. Vis. Exp. (82), e50782, doi:10.3791/50782 (2013).