Pyrosequencing for Microbial Identification and Characterization

Patrick J. Cummings; Ray Ahmed; Jeffrey A. Durocher; Adam Jessen; Tamar Vardi; Kristina M. Obom

doi:10.3791/50405

Journal

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Biology

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미생물 식별 및 특성화를위한 Pyrosequencing

JoVE Journal
Biology

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JoVE Journal Biology

Pyrosequencing for Microbial Identification and Characterization

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미생물 식별 및 특성화를위한 Pyrosequencing

46,274 Views

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12:37 min

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August 22, 2013

DOI:

10.3791/50405-v

DOI:

10.3791/50405-v

•

12:37 min

August 22, 2013

•

19 Views

Patrick J. Cummings¹, Ray Ahmed², Jeffrey A. Durocher², Adam Jessen², Tamar Vardi², Kristina M. Obom¹

¹Center for Biotechnology Education, Krieger School of Arts and Sciences,Johns Hopkins University, ²Qiagen Sciences, Inc.

Summary

Pyrosequencing은 박테리아를 식별하는 균주를 구별하고, 항균 에이전트에 저항성을 부여하는 유전자 변이를 감지하는 데 사용할 수있는 미생물 게놈 시퀀싱을 용이하게하는 다양한 기술입니다. 이 비디오에서는, 미생물 amplicon의 생성,의 amplicon의 pyrosequencing 및 DNA 염기 서열 분석을위한 절차를 설명합니다.

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Pyrosequencing Microbial Identification Microbial Characterization Bacterial Species Identification Bacterial Strain Discrimination Antimicrobial Resistance Detection High-throughput Screening Microbial Genome Sequencing Single Base Sequencing Chemiluminescent Detection Real-time Sequencing SNP Typing Mutation Detection

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미생물 식별 및 특성화를위한 Pyrosequencing

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Summary

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