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September 08, 2012
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CRISPR / CAS系统介导的适应性免疫中的细菌和古细菌。提出了许多CA蛋白作为核糖核酸内切酶作用于不同长度的crRNA前体。在这里，我们说明了三种不同的方法，CAS核酸内切酶活性的生化分析以产生前crRNA的底物。
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Substrate GenerationEndonucleasesCRISPR/Cas SystemsVirusesProkaryotic HostsBacterial EvolutionArchaeal LifeRestriction ModificationAbortive InfectionAdaptive Immune SystemsCRISPR SequencesCas GenesCas ProteinsSpacer ElementCRISPR ClusterPrecursor-crRNACas6 FamilyCrRNAsViral DNA UptakeCascade ComplexBase ComplementarityNuclease Cas3Invader DNA Destruction
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Zoephel, J., Dwarakanath, S., Richter, H., Plagens, A., Randau, L. Substrate Generation for Endonucleases of CRISPR/Cas Systems. J. Vis. Exp. (67), e4277, doi:10.3791/4277 (2012).
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