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Affinity Utfällning av aktiva Rho-GEFs Använda en GST-märkt Mutant Rho Protein (GST-RhoA (G17A)) från epitelceller Lysaten

JoVE Journal
Biology

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JoVE Journal Biology

Affinity Precipitation of Active Rho-GEFs Using a GST-tagged Mutant Rho Protein (GST-RhoA(G17A)) from Epithelial Cell Lysates

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Affinity Utfällning av aktiva Rho-GEFs Använda en GST-märkt Mutant Rho Protein (GST-RhoA (G17A)) från epitelceller Lysaten

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11:28 min

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March 31, 2012

DOI:

DOI:

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11:28 min

March 31, 2012

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Faiza Waheed², Pamela Speight², Qinghong Dan², Rafael Garcia-Mata, Katalin Szaszi²

¹Keenan Research Centre, Li Ka Shing Knowledge Institute,St. Michael’s Hospital , ²Department of Surgery,University of Toronto, ³Department of Cell and Developmental Biology,University of North Carolina at Chapel Hill

Tags

Affinity Precipitation Rho-GEFs GST-tagged Mutant Rho Protein Cytoskeleton Regulation Cellular Processes GTPases Guanine-nucleotide Exchange Factor Pathway-specific Regulation Regulatory Networks Active Pool Of GEFs Affinity Precipitation Assay Burridge Lab

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