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Intrakranielle Implantation mit anschließender 3D In Vivo Bioluminescent Imaging von Murine Gliome
JoVE Journal
Medicine
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JoVE Journal Medicine
Intracranial Implantation with Subsequent 3D In Vivo Bioluminescent Imaging of Murine Gliomas
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Intrakranielle Implantation mit anschließender 3D In Vivo Bioluminescent Imaging von Murine Gliome

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20,115 Views

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09:46 min

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November 06, 2011

DOI:

10.3791/3403-v

DOI:
10.3791/3403-v

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09:46 min
November 06, 2011

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3 Views
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1Neuro-Oncology Research,Barrow Neurological Institute of St. Joseph’s Hospital and Medical Center, 2Neurosurgery Research Laboratory,Barrow Neurological Institute of St. Joseph’s Hospital and Medical Center

Summary

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Intrakranielle Implantation von GL261-Zellen in C57BL / 6 Mäusen produziert maligne Gliome, dass viele Merkmale der menschlichen Glioblastoma multiforme zu rekapitulieren. Wir verwendeten GL261-Zellen stabil exprimiert Luziferase, damit wir verwenden<em> In vivo</em> Bildgebung zu folgen Tumorprogression. Die Operation und 3D<em> In vivo</em> Bildgebung demonstriert.

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Abdelwahab, M. G., Sankar, T., Preul, M. C., Scheck, A. C. Intracranial Implantation with Subsequent 3D In Vivo Bioluminescent Imaging of Murine Gliomas. J. Vis. Exp. (57), e3403, doi:10.3791/3403 (2011).

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