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October 20, 2011
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1Department of Orthodontics and Maxillofacial Orthopedics,Medical University of Graz, 2Institute of Hygiene, Microbiology and Environmental Medicine,Medical University of Graz, 3Department of Prosthodontics, Restorative Dentistry, Periodontology and Implantology,Medical University of Graz, 4Institute of Plant Sciences,Karl-Franzens-University Graz
Summary
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Se presenta un protocolo para el análisis estructural y de composición del biofilm oral, natural de aparatos de ortodoncia con<em> In situ</emHibridación> (FISH) y la microscopía láser confocal de barrido (CLSM). Muestras orales de biopelículas se obtuvieron de expansores del paladar, el raspado de resina acrílica, copos de su superficie y someterlas a la transformación molecular.
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Klug, B., Rodler, C., Koller, M., Wimmer, G., Kessler, H. H., Grube, M., Santigli, E. Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy. J. Vis. Exp. (56), e2967, doi:10.3791/2967 (2011).
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