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October 20, 2011
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1Department of Orthodontics and Maxillofacial Orthopedics,Medical University of Graz, 2Institute of Hygiene, Microbiology and Environmental Medicine,Medical University of Graz, 3Department of Prosthodontics, Restorative Dentistry, Periodontology and Implantology,Medical University of Graz, 4Institute of Plant Sciences,Karl-Franzens-University Graz
Summary
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Vi presentiamo un protocollo per l'analisi strutturale e compositiva delle risorse naturali biofilm orale da apparecchi ortodontici con<em> In situ</em> Ibridazione (FISH) e la microscopia confocale a scansione laser (CLSM). Campioni di biofilm orale sono stati raccolti da espansori palatali, raschiando acrilica sfalda la loro superficie e riferimento per l'elaborazione molecolare.
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Klug, B., Rodler, C., Koller, M., Wimmer, G., Kessler, H. H., Grube, M., Santigli, E. Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy. J. Vis. Exp. (56), e2967, doi:10.3791/2967 (2011).
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