Journal
/
Immunology and Infection
/
Geração e Rotulagem de murino derivadas da medula óssea Células Dendríticas com Nanocristais Qdot de Estudos de rastreamento
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Generation and Labeling of Murine Bone Marrow-derived Dendritic Cells with Qdot Nanocrystals for Tracking Studies

Geração e Rotulagem de murino derivadas da medula óssea Células Dendríticas com Nanocristais Qdot de Estudos de rastreamento

português

Automatically Generated

24,584 Views

•

07:34 min

•

June 02, 2011

DOI:

10.3791/2785-v

DOI:
10.3791/2785-v

•

07:34 min
June 02, 2011

•

7 Views
, , ,
1Molecular and Cell Biology Program,Ohio University, 2Department of Biomedical Sciences,College of Osteopathic Medicine, Ohio University, 3Department of Biomedical Engineering,Russ College of Engineering and Technology, Ohio University

Summary

Automatically generated

Dendríticas células antígenos captação e migrar para órgãos imunes a antígenos presentes processados ​​às células T. Qdot nanocristais rotulagem fornece um sinal duradouro e estável fluorescente. Este permite o rastreamento de células dendríticas a diferentes órgãos, por microscopia fluorescente.

Explore More Videos

GenerationLabelingMurineBone Marrow-derived Dendritic CellsQdot NanocrystalsTracking StudiesAntigen Presenting CellsImmunological OrgansMajor Histocompatibility MoleculesMigratory CapabilitiesFluorescent DyesQdot Fluorescent NanocrystalsStable FluorescenceCell CultureDifferentiationIn Vitro IncubationFluorescent MicroscopyExperimental AnimalsMature Cells

Read Article

Cite this Article

Muccioli, M., Pate, M., Omosebi, O., Benencia, F. Generation and Labeling of Murine Bone Marrow-derived Dendritic Cells with Qdot Nanocrystals for Tracking Studies. J. Vis. Exp. (52), e2785, doi:10.3791/2785 (2011).

Download .ris file

Copy

Share Video

.

Copy