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Generatie en etikettering van Muriene Bone Marrow-afgeleide dendritische cellen met Qdot Nanokristallen voor Tracking Studies
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Generation and Labeling of Murine Bone Marrow-derived Dendritic Cells with Qdot Nanocrystals for Tracking Studies
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Generatie en etikettering van Muriene Bone Marrow-afgeleide dendritische cellen met Qdot Nanokristallen voor Tracking Studies

Nederlands

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24,584 Views

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07:34 min

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June 02, 2011

DOI:

10.3791/2785-v

DOI:
10.3791/2785-v

•

07:34 min
June 02, 2011

•

7 Views
, , ,
1Molecular and Cell Biology Program,Ohio University, 2Department of Biomedical Sciences,College of Osteopathic Medicine, Ohio University, 3Department of Biomedical Engineering,Russ College of Engineering and Technology, Ohio University

Summary

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Dendritische cellen opname van antigenen en migreren naar immuun organen verwerkte antigenen presenteren aan T-cellen. Qdot nanokristallen etiket zorgt voor een langdurige en stabiele fluorescerende signaal. Dit maakt het volgen van dendritische cellen om verschillende organen van fluorescentie microscopie.

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Muccioli, M., Pate, M., Omosebi, O., Benencia, F. Generation and Labeling of Murine Bone Marrow-derived Dendritic Cells with Qdot Nanocrystals for Tracking Studies. J. Vis. Exp. (52), e2785, doi:10.3791/2785 (2011).

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