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組換え可溶性GM - CSF受容体を用いてGM - CSF抗体の中和能を推定する無細胞アッセイシステム
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Cell Free Assay System Estimating the Neutralizing Capacity of GM-CSF Antibody using Recombinant Soluble GM-CSF Receptor
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組換え可溶性GM - CSF受容体を用いてGM - CSF抗体の中和能を推定する無細胞アッセイシステム

日本語

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12,611 Views

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07:45 min

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June 27, 2011

DOI:

10.3791/2742-v

DOI:
10.3791/2742-v

•

07:45 min
June 27, 2011

•

1 Views
, , , , , , ,
1Bioscience Medical Research Center,Niigata University Medical and Dental Hospital, 2First department of Internal Medicine, School of Medicine,Kyorin University, 3Neosilk Laboratory,Immuno Biological Laboratories Co., Ltd.

Summary

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我々は、受容体への顆粒球マクロファージコロニー刺激因子（GM - CSF）の結合を推定するために、無細胞受容体結合アッセイをデザインしました。それは、優れた再現性とGM - CSF自己抗体による可溶性GM - CSF受容体αに結合するビオチン化GM - CSFの競合的阻害を評価することを可能にします。

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Cite this Article

Urano, S., Tazawa, R., Nei, T., Motoi, N., Watanabe, M., Igarashi, T., Tomita, M., Nakata, K. A Cell Free Assay System Estimating the Neutralizing Capacity of GM-CSF Antibody using Recombinant Soluble GM-CSF Receptor. J. Vis. Exp. (52), e2742, doi:10.3791/2742 (2011).

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