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February 17, 2011
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This communication presents data on the accuracy and reproducibility of the BioSpec-nano UV-VIS spectrophotometer for dsDNA and protein quantitation. Even with ultra-small volumes (1 to 2 L), reproducibility is excellent, while the automated wiping function improves throughput and results in minimal carryover for more precise results.
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Concentration DeterminationNucleic AcidsProteinsMicro-volumeBio-spec Nano SpectrophotometerQuantitation ProceduresMolecular BiologistsSmall-volume AnalysisAccurate ResultsDNA Quantitation MethodsSpecialized OpticsPDA DetectorAutomated FunctionalityMeasurement ReproducibilityMeasurement AccuracySample ContactRapid ScanningThroughputData AnalysisSoftware FeaturesDNA Concentration CalculationSample Dilution FactorNucleic Acid Concentration FactorProtein Concentration Results
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Sukumaran, S. Concentration Determination of Nucleic Acids and Proteins Using the Micro-volume Bio-spec Nano Spectrophotometer. J. Vis. Exp. (48), e2699, doi:10.3791/2699 (2011).
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