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05:23 min
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May 10, 2011
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ここではのラベル付けを可能にする手順を説明します。<emその場で>エドワードシエラ属ictaluri</em>ブチナマズから組織切片で<em> Ictalurus punctatus</em>セカンダリとして、プライマリ、および蛍光FITC標識抗体としてモノクローナル抗体を用いた間接免疫組織化学Ed9を使用。これは、蛍光顕微鏡を用いた細菌の検出に使用できます。
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Fluorescent Immuno-ChemistryDetectionEdwardsiella IctaluriChannel CatfishIctalurus PunctatusMethod DevelopmentIn Situ VisualizationBacteriaHistological SectionsBacterial LocalizationPlate CountsRadiometric LabeledBioluminescent BacteriaGross Organ LevelInfection CycleVirulence FactorsInteraction With HostSalmonella TyphiTaxonomic FamilyTechnique DescriptionIndirect Immuno-histochemistryMonoclonal Ed9 AntibodyBlocking SerumParaffin Embedded Histological SectionsPrimary AntibodySecondary AntibodiesFitC Labeled Secondary Antibodies
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Menanteau-Ledouble, S., Lawrence, M. Use of Fluorescent Immuno-Chemistry for the detection of Edwardsiella ictaluri in channel catfish (I. punctatus) samples. J. Vis. Exp. (51), e2687, doi:10.3791/2687 (2011).
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