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August 04, 2011
DOI:
1Interdepartmental Program in Translational Biology & Molecular Medicine,Baylor College of Medicine, 2Texas Children's Cancer Center,Baylor College of Medicine, 3Department of Pediatrics,Baylor College of Medicine, 4Department of Pathology,The Methodist Hospital Research Institute, 5Department of Ophthalmology,Retinoblastoma Center of Houston, 6Baylor College of Medicine,Center for Cell and Gene Therapy, 7Center for Cell and Gene Therapy,Baylor College of Medicine
Summary
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Se describe un método para propagar los tumores humanos en ratones retinoblastoma. Las células tumorales se inyectan directamente en los ojos de ratones inmunes deficientes. Los tumores secundarios se han establecido con éxito utilizando las células cosechadas directamente de tumores humanos y tumorspheres cultivadas.
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Bond, W. S., Wadhwa, L., Perlaky, L., Penland, R. L., Hurwitz, M. Y., Hurwitz, R. L., Chèvez-Barrios, P. Establishment and Propagation of Human Retinoblastoma Tumors in Immune Deficient Mice. J. Vis. Exp. (54), e2644, doi:10.3791/2644 (2011).
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