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Mutagenese und Functional Selection Protocols for Directed Evolution von Proteinen in E. coli</em
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Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
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Mutagenese und Functional Selection Protocols for Directed Evolution von Proteinen in E. coli</em

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29,879 Views

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09:01 min

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March 16, 2011

DOI:

10.3791/2505-v

DOI:
10.3791/2505-v

•

09:01 min
March 16, 2011

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10 Views
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1Department of Microbiology & Environmental Toxicology,University of California Santa Cruz – UCSC

Summary

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Hier zeigen wir ein einfaches Protokoll, um eine zufällige Mutantenbibliothek für eine bestimmte Zielsequenz zu schaffen. Wir zeigen, wie diese Methode, die in vivo in Escherichia coli durchgeführt wird, mit funktionellen Selektionen gekoppelt werden kann, um neue enzymatische Aktivitäten entwickeln.

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Troll, C., Alexander, D., Allen, J., Marquette, J., Camps, M. Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli. J. Vis. Exp. (49), e2505, doi:10.3791/2505 (2011).

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