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13:45 min
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December 23, 2010
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Summary
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本論文では、特定のリガンドへの白血球の走化性応答を決定し、生細胞イメージング技術を用いて細胞表面の受容体と細胞質のタンパク質間の相互作用を識別するための方法論を説明します。
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Leukotriene B4Cell MigrationBLT1β-arrestinG-protein Coupled ReceptorsTransmembrane Protein FamilyExtracellular SignalsIntracellular ResponsesChemotaxisCalcium ReleaseGene RegulationLigand BindingConformational ChangesHeterotrimeric G-proteinsSecond MessengersCyclic Adenosine Monophosphate (cAMP)Inositol Triphosphate (IP3)Diacyl Glycerol (DG)DesensitizationSequestrationInternalizationG-protein Receptor Kinases (GRKs)Cytosolic ProteinsMembrane TranslocationPro-inflammatory Lipid MoleculeArachidonic Acid Pathway
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Jala, V. R., Haribabu, B. Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with β-arrestin. J. Vis. Exp. (46), e2315, doi:10.3791/2315 (2010).
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